Alpha Teknova, Sartorius BIA Separations share data on AEX platform

Alpha Teknova and Sartorius BIA Separations shared new data that demonstrates the ability to improve the recovery of full AAV capsids during anion-exchange, AEX, purification, which will help gene therapy companies streamline the downstream processing workflow. Teknova presented the findings from the companies’ first collaboration earlier this week during a presentation entitled, “Enhancing AAV Full Capsid Enrichment with an Optimized AEX Platform: A Case Study.” After identifying capsid enrichment as a critical pain point in the AAV gene therapy workflow, Teknova and Sartorius BIA Separations began working together to address the challenges gene therapy developers face. By using a robust design of experiments approach, the companies were able to achieve more than 85 percent full capsid enrichment with 80-95 percent recoveries using custom Teknova buffers along with the Sartorius BIA Separations CIMmultus QA monolith for the polishing step, given a specific starting material of AAV8 that contained 46 percent full capsids. The buffers and parameters discovered in this study were then further optimized to create a first-of-its-kind product available off-the-shelf for gene therapy developers: Teknova’s AAV-Tek AEX Buffer Screening Kit for AAV8.